WebPCR, using a standard Taq DNA polymerase without a hot start. Analysis was carried out on the Agilent 2100 bioanalyzer using the DNA 1000 LabChip kit. Use of standard Taq DNA polymerase results in the generation of large amounts of primer-dimers and low efficiency of amplification. In contrast, the stringent hot start provided by HotStarTaq DNA WebThe quantity and quality of total RNA was subsequently analyzed using a NanoDrop 1000 spectrophotometer (Peqlab, Erlangen, Germany) and RNA 6000 Pico chips on an Agilent 2100 Bioanalyzer (Agilent Technologies, Böblingen, Germany). ... libraries were again purified using the method described above and the size distribution was assessed using ...
DNA Sequencing Facility The University of Edinburgh
WebItem Agilent DNA 1000 Kit Company Agilent Technologies Price Get Quote Get Quote for this Product Catalog Number 5067-1504 Agilent Technologies 5301 Stevens Creek Blvd Santa Clara, California 95051 United States Phone: +1 877-424-4536 Company Profile Website: www.agilent.com Citations (181) Websamples with approximately 1000 cells3. Although the Agilent 2100 bioanalyzer is a reliable tool in RNA quality control, RNA quanti- ... and sheared genomic DNA (slightly elevated general ... rich n shit bpm
IJMS Free Full-Text DNA Demethylation of Myogenic Genes …
Web文库长度分布检测,可通过Agilent Bioanalyzer 2100等基于毛细管电泳或微控流原理的设备进行检测。 ... 使用 Hieff NGS ® Ultima DNA Library Prep Kit for Illumina ® 对 100 ng FFPE样本建库(未分选),结果使用Agilent 2100 DNA 1000 Chip ... WebApr 13, 2024 · The T-DNA insertion was confirmed by genomic PCR using the ... and subjected to quality control using an Agilent 2100 Bioanalyzer. Libraries were prepared using a TruSeq stranded messenger RNA ... WebEquipment Required for a DNA 500 or DNA 1000 Assay Equipment supplied with the Agilent 2100 bioanalyzer •Chip Priming Station (reorder number 5065-4401) •IKA Vortex mixer (optional) Additional material required (not supplied) •Pipettes (10 µl, 100 µl and 1000 µl) with compatible tips •0.5 ml microcentrifuge tubes for sample preparation red rooster employment age