2024 Biotin-coupled mirna capture

Biotin-coupled mirna capture

Author: gyjb

August undefined, 2024

WebNov 23, 2024 · Biotin-coupled miRNA capture, fluorescence in situ hybridization and luciferase reporter assays were conducted to confirm the relationship between circMMP9 and miR-124. In this study, we screened differentially expressed circRNAs and identified circMMP9 in GBM. We found that circMMP9 acted as an oncogene, was upregulated in … WebMay 31, 2024 · The biotin-coupled miRNA capture assay was performed as previously described. 17 In brief, miR-206 mimics and miRNA negative control were labeled with biotin at the 3′ end and transiently transfected into LoVo and HCT116 cells. After 24 hrs of culture, the cells were harvested and lysed in a lysis buffer, followed by incubation with ...

Circular RNA circHIPK3 promotes cell proliferation …

WebFeb 10, 2024 · Biotin-coupled miRNA capture. The 3′ end biotinylated miR-578 or control mimic RNA (RiboBio) was transfected into RF/6A cells for 24 h at the concentration of 30 nM. The biotin-conjugated RNA complex was pulled down by incubating the cell lysates with streptavidin-coated magnetic beads (Life Technologies). The amount of cZBTB44 in … WebJul 13, 2024 · 2.13 Biotin-coupled miRNA capture. The biotin-coupled miRNA pull-down assay was carried out as previously described. 17, 18 Briefly, the 3′-end biotinylated miR-RNA mimic or control biotin-RNA (RiboBio) was transfected into SPCA1 cells at a final concentration of 20 nmol/L for 1 day. The biotin-coupled RNA complex was pulled … medicare medicaid number for physicians

circRNA-002178 act as a ceRNA to promote PDL1/PD1 expression …

WebDec 12, 2024 · Streptavidin coupled to a solid matrix of beads allows the specific capture of biotinylated RNA easily. To demonstrate the suitability of biotinylated cap analogues for the study of protein–RNA interactions we established a combined protocol including ultraviolet (UV) cross-linking, and affinity purification of biotinylated-capped RNA. WebMay 15, 2024 · They found that miR-193a-3p functioned as a tumor suppressor in PCa cells, through bioinformatic prediction and biotin-coupled miRNA capture studies. Functional studies showed that miR-193a-3p reduced MCL1 expression, to suppress proliferation, migration, and invasion in cancer cells . medicare medicaid masshealth

Capture and Identification of miRNA Targets by Biotin

circCHST15 is a novel prognostic biomarker that promotes clear …

WebMiR-4306 response elements were predicted by bioinformatics algorithm and identified using dual-luciferase reporter, RNA immunoprecipitation, and biotin-coupled miRNA capture assays. Results CircPDE4D was markedly downregulated in OA cartilages and interleukin (IL)-1β-stressed human normal chondrocytes (HNC). WebJul 17, 2024 · Biotin-coupled miRNA capture. Because PTEN plays an important role in regulating cell proliferation and is one of the miR-181b-5p target genes, a pull-down assay was performed to investigate whether miR-181b-5p directly binds to PTEN. Briefly, HPDLSCs were transfected with biotinylated miRNA mimics or nonsense control (NC) … medicare medicaid low incomeWebOct 23, 2024 · Biotin-coupled miRNA capture. The biotin-coupled miRNA pull-down assay was performed as described previously by … medicare medicaid nursing home 07860

"WebNov 9, 2024 · On the contrary, some studies argued that biotin moiety when attached to the 3′ end of the miRNA impede its association with AGO2. 41 Hence, biotin-labelled miRNA was not co-IPed with AGO2. 28 The negative result could be due to low transfection efficiency or post-translational modification of AGO2 that can hamper miRNA-AGO2 … " - Biotin-coupled mirna capture

Biotin-coupled mirna capture

The CircRNA-ACAP2/Hsa-miR-21-5p/ Tiam1 Regulatory Feedback …

WebIn the biotin-coupled miRNA capture assay, the direct interaction between HIPK3 and miR-194 was verified. While upregulating HIPK3 promoted NSCLC cell proliferation, co-transfection with both HIPK3 and miR-194 abrogated the tumor-promoting effects of HIPK3. Altogether, these data corroborated that HIPK3 and miR-149 exert opposing roles in … WebJan 31, 2024 · The biotin-coupled RNA complex was pull-downed by incubating the cell lysates with streptavidin magnetic beads (Life Technologies, USA) for another 4 h. The …

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WebFeb 27, 2013 · Biotin-coupled miRNA capture Biotin-labelled miRNAs were transfected into HEK293 cells at a final concentration of 20 nM. Forty-eight hours after transfection, cells were collected and treated as ... WebMay 26, 2024 · Biotin-coupled miRNA capture. The 3′ end of biotinylated miR-556-5p and miR-1322 mimics or control RNA (Ribio, Guangzhou, China) were transfected into 1 × 10 6 HCT116 and DLD1 cells at a final concentration of 50 nM for 48 h before harvest. Then the cell pellet was incubated with 0.7 mL lysis buffer (5 mM MgCl 2. In vivo experiment

WebJul 19, 2024 · Bioinformatic analysis was utilized to predict potential miRNA target sites, and biotin-coupled miRNA capture, biotin-coupled probe pull-down assay, and RNA … WebInvitrogen streptavidin-coupled Dynabeads are a robust and versatile tool that can be used to capture specific RNA or DNA sequences and then pull them directly out of solution. …

WebJan 16, 2024 · RNA immunoprecipitation (RIP) and biotin-coupled miRNA capture. g for 20 min. Western blot analysis. The total protein of 95D cells were exacted using protein extraction reagent (Thermo Scientific ... WebDec 20, 2024 · Biotin-coupled miRNA capture. For biotin-coupled capture, approximately 5 × 10 6 BCa cells (T24 and 253 J-BV) were transfected with biotin-miR135a-5p or negative control (NC) by …

WebJan 15, 2024 · Biotin-coupled circRNA and miRNA capture. Biotin-coupled miRNA and circRNA pull-down assays were performed as described previously [11, 12]. Briefly, 3′ end biotinylated circRNA-ACAP2 (digoxin-5′-TCACACAGGCAGCTTTGGAAGAAG-3′-Digoxin; RiboBio, Guangzhou, China) was transfected into SW480 cells at a final concentration of …

WebDec 18, 2024 · Biotin-coupled miRNA capture. The 3′ end biotinylated miRNA mimics or control RNA (Ribio, Guangzhou, China) were transfected into ccRCC cells for 48 h before harvest. Then biotin-coupled RNA complex was pulled down by incubating the cell lysates with streptavidin-coated magnetic beads (Life Technologies). The abundance of … medicare medicaid for seniorsWebJan 16, 2024 · RNA immunoprecipitation (RIP) and biotin-coupled miRNA capture. g for 20 min. Western blot analysis. The total protein of 95D cells were exacted using protein extraction reagent (Thermo Scientific) with a cocktail of proteinase inhibitors (Roche Applied Science, Switzerland). Equal amount of total protein was separated by 10% sodium … medicare medicaid insurance plansWebJun 19, 2024 · Biotin-coupled miRNA capture. The biotin-coupled miRNA pull-down assay was performed as previously described 11. Briefly, 3′-end biotinylated miR-587 mimics (Ribio, Guangzhou, China) were ... medicare medicaid nursing facilitiesWebJun 19, 2024 · Biotin-coupled miRNA capture. The biotin-coupled miRNA pull-down assay was performed as previously described 11. Briefly, 3′-end biotinylated miR-587 mimics (Ribio, Guangzhou, China) were ... medicare medicaid office addressWebFeb 27, 2013 · Biotin-coupled miRNA capture Biotin-labelled miRNAs were transfected into HEK293 cells at a final concentration of 20 nM. Forty-eight hours after transfection, cells were collected and treated as ... medicare medicaid pay for inogenWebJan 10, 2024 · Biotin‐coupled miRNA capture The 50 μ m biotinylated miRNA mimic (GenePharma, Shanghai, China, sequence GCCCTTCATGCTGCCCAG) was … medicare medicaid official websiteWebThe intracellular transfer of circEDIL3 by SMSCs-Exos may be a potential novel therapeutic strategy for Rheumatoid Arthritis. medicare medicaid office jobs