Cre reporter tool strain
WebResults. Throughout this study, we utilize the Ai14D reporter mouse strain (Madisen et al., 2010; Kauffman et al., 2024) to assay Cre activity throughout the body (Figure 1A).This reporter carries a transgene at the ‘safe harbor’ Rosa26 locus, with a ubiquitously expressed CAG promoter driving expression of a transgene with a 3× STOP cassette … WebAug 1, 2024 · The Cre-loxP recombination system derived from bacteriophage P1 is one of the most popular site-specific recombination tools. ... [10] developed a set of Cre reporter mice, including the Ai14 strain, that employed a previously reported STOP cassette containing three identical 254 bp poly(A) signal repeats derived from the SV40 virus [11].
Cre reporter tool strain
Did you know?
WebTools. Data Hub. Learn more about Addgene materials from user-contributed reports describing AAV and antibody experiments. ... Generalized lacZ expression with the ROSA26 Cre reporter strain. Soriano P Nat Genet. 1999 Jan . 21(1):70-1. PubMed Article Plasmids from Article. ID Plasmid Purpose ; 13444: PGKneotpAlox2: WebThe R26R mouse strain should be of wide use for monitoring Cre expression, as well as for analysing cell lineages during development, and is available from the Induced Mutant Resource of the ...
WebApr 27, 1999 · Effective use of conditional Cre recombinase-loxP gene modification requires Cre-expressing mouse strains with defined patterns of expression.To assess the in vivo … WebBackground: Several Cre reporter strains of mice have been described, in which a lacZ gene is turned on in cells expressing Cre recombinase, as well as their daughter cells, …
WebAug 7, 2024 · New reporter mouse strain offers powerful genetic tool to identify P2X2-expressing cells by University of South Florida tdTomato (red) expression in nodose …
WebMay 19, 2016 · The Cre-loxP–dependent expression of certain genetic markers in a specific subset of cells is also a powerful tool for analyzing cell lineages and tracing transplanted …
WebMar 27, 2001 · The Cre-loxP site specific recombination system [] is widely used for production of tissue-specific and conditional knockout alleles in mice [2,3].Recently, a … population calculator growthWebAug 7, 2024 · So, the USF Health researchers created a knockin mouse incorporating a powerful genetic approach that could be used in future experiments. They made a mouse that expresses the bacterial enzyme … population cache valley utahWebOct 26, 2024 · This reporter system produces differentially labeled mutant and wild-type cells from any Cre/loxP-based conditional alleles in the mouse. We then tested the utility of MASCOT in the study of hematopoiesis and CH. shark steamer handheld manualWebprogeny resulting from a cross between this reporter line and K5-Cre transgenic mice, in which Cre is driven by a keratin-ocyte-speci¢c promoter [13]. These results indicate that this reporter strain should be a useful tool for monitoring the Cre expression driven by various promoters in transgenic mouse lines. 2. Materials and methods 2.1. Mice population calgary 2022WebJun 22, 2024 · Cre recombinase is a 38-kDa protein originally discovered in bacteriophage P1 [ 11] that mediates site-specific intermolecular DNA recombination between two loxP sites. Cre recombinase recognizes the loxP sequences flanking a targeted DNA sequence and creates a DNA loop. shark steamer mopWebThe JAX Cre Repository uses a lacZ Cre-reporter strain, B6.129S4-Gt(ROSA)26Sor tm1Sor/ J, to assess cre activity. β-Galactosidase staining of 10.5 dpc embryos is done … For Cre characterization work at the Jackson Laboratory, there is a 7‐day … The Jackson Laboratory Cre Repository uses whole-mount β-galactosidase … The Knockout Mouse Project (KOMP) is providing critical tools for understanding … Each new mutant strain is characterized genetically, genomically and … population calgaryWebMar 9, 2024 · A reporter mouse strain expressing ACR1 under the control of Cre recombinase was reported recently 13, but a reporter mouse strain expressing ACR2 … shark steamer instructions