WebIf you wish to narrow the excitation band for blue excitation, you might choose an interference excitation filter (very sharp slopes on either side of the excitation band) BP460, a dichroic mirror DM505 and a barrier long pass 515IF (interference barrier filter). The sharp slopes of the exciter and barrier filters do a better job in separation ... WebJun 2, 2024 · For APC, the excitation source can vary depending on the available lasers. The 594 nm or 633 nm laser line are suitable. For emission filters, the Cy5 filter (660/40) is recommended. Determine if compensation is necessary For multicolor analysis, compensation may be required before analyzing data.
Alexa Fluor Dyes—Across the Spectrum - Thermo Fisher …
WebWavelengths of maximum absorption (≈ excitation) and emission (for example, Absorption/Emission = 485 nm/517 nm) are the typical terms used to refer to a given fluorophore, but the whole spectrum may be important to consider. The excitation wavelength spectrum may be a very narrow or broader band, or it may be all beyond a … WebA version of the 89000 Quad set with a quad band excitation filter, dichroic and emission filter. Useful for simultaneous live viewing of multiple channels or image-splitting appl new fame
Cyanine - Wikipedia
Web49009 - ET - Cy5 Narrow Excitation. Price: $825.00. The narrow-band excitation provided by this filter set is useful in applications where fluorochromes such as Texas Red or … WebCy5 Filter Set This Iridian single band filter set consists of the 628-40 EX, 692-40 EM and 660 DM filters. It is optimised for the cyanine dye Cy5. The set contains excitation, emission and dichroic filters designed to fit in standard filter cubes from the leading microscope and third-party manufacturers. Iridian website Related WebThe excitation channels are driven by a motor that can automatically switch the two excitation channels for taking images of up to seven different fluorescent dyes. An algorithm to calibrate the crosstalk between different fluorescent dyes is also provided. Also provided is a method for analyzing digital PCR data using a ratio of two ... new family addition