WebFeb 3, 2024 · Here, we report an easy and efficient method to synthesize functional mRNAs by modifying multiple 5′ cap analogs using a vaccinia virus-capping enzyme. We show that this enzyme can introduce a variety of GTP analogs to the 5′ end of RNA to generate 5′ cap-modified mRNAs that exhibit different translation levels. WebApr 29, 2024 · Double endcapping means it is endcapped twice. Double (or triple) endcapping further minimizes silanol interactions and improves stability at mid pH. When developing methods in the mid pH range, it is recommended to use an endcapped column. As pH increases, silica dissolution is decreased by endcapping.
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