Witryna19 paź 2016 · A new SLK cell line was established to ectopically express RTA (SLK-RTA) as a Notch signaling donor. Consistently, JAG1 was up-regulated in SLK-RTA cells compared with control cells (SLK-Ctrl) ( S2A Fig ). iSLK.RGB cells, as the Notch signaling recipient, were co-cultured with SLK-Ctrl or SLK-RTA cells for 24 and 48 h. Witryna28 maj 2024 · The iSLK cells (1 × 10 6 cells) were transfected using the calcium phosphate method. The transfected cells were selected under 1000 μg/mL of hygromycin B (Wako, Osaka, Japan) to establish doxycycline-inducible recombinant KSHV-producing cell lines (iSLK-WT, iSLK-FS-ΔORF7, iSLK-ST-ΔORF7, iSLK-FS …
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Witryna1 mar 2011 · A workhorse cell line for KSHV research, iSLK cells have been engineered to express the viral lytic switch protein, RTA, in a doxycycline (Dox)-inducible manner (69). These cells are... Witryna2 gru 2024 · Direct knockdown of HxRs in iSLK.219 cells effectively blocked viral lytic gene expression during induction. Using samples from a cohort of HIV+ patients, we … most valued employee benefits
Infectious BAC16 virus production. A BAC16 virus stock
Witryna16 kwi 2024 · Transcriptome-wide m6A-sequencing of the KSHV-positive renal carcinoma cell line iSLK.219 during lytic reactivation revealed the presence of m6A across multiple kinetic classes of viral transcripts, and a concomitant decrease in m6A levels across much of the host transcriptome. WitrynaThe first KSHV genome was mapped in 1996, with phage and cosmid libraries from the lymphoid BC-1 cell line (GenBank ID: U75698.1) ... unknown genomic and functional features were assessed using a tightly controlled and highly inducible epithelial iSLK-219 cell line containing a doxycycline inducible transgene that encodes viral RTA protein. Witryna17 sty 2024 · In brief, KSHV lytic replication was induced in an iSLK-puro cell line that harbours the KSHV-BAC16 plasmid 31, 32. The tissue culture supernatant was collected and KSHV virions were pelleted... most valued employee benefits uk